High-Throughput qPCR Microfluidics Technology With Singleplex Accuracy

Presenter: Naveen Ramalingam, PhD
Senior Director of Assay Development at Standard BioTools™

Abstract:

→ Problem
Screening greater than 10 pathogens or SNPs in a sample is challenging due to limitations in fluorescence colors in a multiplex quantitative PCR. Optimizing a multiplex qPCR is daunting, and it limits addition or removal of assays without optimizing again.

→ Solution
Microfluidics qPCR technology allows massively parallel reactions using a single color with singleplex accuracy. The sensitivity and sample input volume are comparable to those of a standard thermal cycler workflow. Miniaturization leads to cost saving, simplified high-throughput workflow and efficient reaction kinetics.

→ Proof
The sensitivity of microfluidics qPCR technology is comparable to that of a standard qPCR thermal cycler. It can generate 9,216 qPCR results in 4 hours.

→ Learning Objectives
Understand how microfluidics enables high-throughput qPCR with singleplex accuracy.
Understand the impact of miniaturization on sample and reagent volume savings.
Learn how microfluidics technology addresses challenges of multiplex qPCR.