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Microfluidics: How it Works

Every Fluidigm IFC contains a network of fluid lines, chambers and Nanoflex™ valves—rubber membranes that deflect under pressure to pinch off the flow of fluids in a microchannel. The movement, mixing and reactions of samples and reagents are controlled within the chambers of the IFC by use of microscopic gates, resulting in a closed system that eliminates many individual pipetting steps and provides low contamination risk with high reliability. Samples and assays are loaded separately and then combined in a pairwise manner to create unique individual reactions.